Desktop.ini is a Windows system file that stores information about customized folders. You can customize folder background, change icon or thumbnail image to a folder and create infotip by creating a Desktop.ini file for that folder.
Desktop.ini is a hidden file. It means that you could see it in windows file manager only if you checked 'Show hidden files and folders' in Folder Options.
Desktop.ini can be deleted, but any view customizations related to the folder containing the file in question, will be reset to the system-wide default.
Note: Any malware can be named anything - so you should check where the files of the running processes are located on your disk. If a "non-Microsoft" .exe file is located in the C:Windows or C:WindowsSystem32 folder, then there is a high risk for a virus, spyware, trojan or worm infection!
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